Pepper Party

Pepper Party

Thursday, April 17, 2014

science thursday

I got the plague last weekend after all. Now we're all better! We have a new sofa, clean carpets, and lots of pictures for you!

I realized, again, this week while I was dithering about trying an important experiment, that the point of most important experiments is that you genuinely don't know the outcome. You think and hope that it will go one way, but you don't know! It's why you have to do the experiment! But for many experiments in molecular biology, the techniques are routine. If you want to clone a gene, and you know anything about the gene, the tools and methods are all standardized and you should be able to just make it happen. The hard part is moving beyond the routine data-collection and DNA manipulation to try something new that will expand the field.

It reminds me of when I was little and had long hair. I was very good at brushing the front bits that I could see and that had no knots in them. The back, though, was a territory I did not like to venture into!

It's hard to do the big experiments, too, because they are often expensive or involve some precious reagent. So if your protocol doesn't yield the answer you hope for, and you want to try again by varying the protocol a little bit, well, you might be out of luck. My instinct becomes waiting to see if the protocol will work perfectly, and then try the big experiment - which means it just takes forever for me to get around to doing it! In other words, the front bits of my hair are always very thoroughly detangled.

We'll see tomorrow how the experiment goes - until then here are some photos:




Oscar takes after his da in many ways, but up until now he hasn't been too keen on hats. The problem, it seems, is that he can't put them on his head himself. But this hat he figured out the trick to, and it made him very happy to put it on and take it off again!


Oscar is almost able to appreciate pictures of himself. While Eric was cleaning the carpets this weekend I entertained the boy by taking videos of him and playing them back. He watched them with fierce concentration, but only mimicked himself when he was doing something wicked like blowing raspberries.


Our new sofa, in its closed conformation. We laid on the bed last night and watched Columbo. It was extremely comfortable, once we got the plastic off the mattress. I was tempted to leave it on for all time (permanently free of cat hair!) but comfort won out almost instantly.



Look at him handling that banana all by himself! He's almost a pro! He's still working on learning how to manage the peel. He often licks it, and makes a terrible face. (Can you imagine being a professional banana-eater? -- Never speak of this.)

More! All Done!




Convinced? It's not a perfect system, because he signs "more" whenever he sees a banana. Or, who knows, maybe he loves bananas that much!

Thursday, April 10, 2014

a plague on both your parents!

Oscar has given Eric the plague, so Oscar now feels perfectly happy and Eric is miserable. I'm holding out so far.
On Friday Oscar went in for his checkup. 27 pounds, 33.5 inches. That is, he's almost 3 feet tall! He's exactly half as tall as me! He's at the top of the charts for both stats, too.

Daffodils have started blooming, and some other pretty blue flowers (not squill) that I got on a whim in the fall. I have no clue what they are, except pretty. There's one lonely hot pink tulip getting ready to open too. The daylilies are emerging, as are the peonies and the beebalm. All the roses are making leaves, even the one we transplanted from the side of the house to the side of the shed, which seemed like it kind of wanted to die after that. I saw one little hint of a leaf on the peach tree today, so of course the forecast is for snow this weekend.


Eric's bonsai is really happy all of a sudden!


I made a lemon Bundt cake this weekend - it's almost gone now. Eric pretends this isn't cake, so at least I didn't eat it all by myself. It is full of lemon essence. Here is the recipe.

Oscar is a thrill-seeker and a nut-job.



Wednesday, April 2, 2014

What's new, hmmm?

What's new, hmmm?

Last Friday I got 3 liters of liquid nitrogen for freezing my Arabidopsis samples. I collected the samples and froze them, and they came with me in the liquid nitrogen dewar to Plant SuperGroup. Then me and my buddies went all the way across campus to the freeze-dryer and they started drying out. This operation made me and Eric late to pick up Oscar. Fortunately, we had a dewar full of liquid nitrogen to share! Becky and Lindsay and Rachel got to try freezing candy instantly, and then waited for it to thaw before they ate it. They also froze a hot dog and smashed it to bits on the deck. Unfortunately, Becky's siliconized tongs they were using to hold the hot dog smashed to bits also. Oh, well. We'll get her a replacement. Then Eric took the rest of the liquid and dumped it in the gravel pit. A thick fog spread over the ground, and we got out of there!

I took a writing class this semester, in anticipation of being asked to be a TA for a new upper-division undergraduate class incorporating lots of writing. And I will be that TA! We were doubtful that the instructional committee would permit this particular class, Plant Physiology, to have the intensive-writing section (a Capstone course), but they have decided to allow it after all. I will get some summer salary to help with the curriculum design, and next spring presumably I will get to teach it. This may be the last semester I TA Cell Biology...

Eric is learning all about radiolabeling plants and tracing the radioactivity. He's hot! (Or he will be if he's not careful.)

Finally, we have not imaged Oscar lately, we're sorely behind in our data collection on that front, but here is a picture of me with a Punjar (Swedish jackhammer), from when I was in ACE. That thing doesn't mess around!